ENVIRONMENTAL SCIENCE
Cat. #951 Chromogenic Analysis of Water Contaminants
NIH Grant #SBIR-IR44-RR018670. Opinions expressed are those of the authors and not necessarily those of the NIH/NCRR.
For 10 Groups. Testing drinking water for every possible type of pathogenic bacteria is slow and costly. Thus, drinking water is tested for coliforms - including the familiar E. coli . Presence of coli- forms is an indicator of fecal contamination. In this experiment, students will test for coliforms in simulated contaminated water using color and fluorescent reagents. They can use these same reagents to test water samples from the envi - ronment. As an extension activity, a Gram Stain test can be performed on the collected samples.
Complete in 30 min. and grow overnight.
Storage: Some Components Require Refrigerator Storage. CLICK HERE For Experiment #951 Components and Requirements.
PERFECT PARTNER
Cat. #969 Long Wave UV Light
Cat. #953 Multiplex PCR-based Testing of Water Contaminants
For 25 Students. Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experi- ment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
NIH Grant #SBIR-IR44-RR018670. Opinions expressed are those of the authors and not necessarily those of the NIH/NCRR.
Extraction 60 min. PCR Set Up 10 min.
PCR 2 hrs. to overnight Electrophoresis 60 min. Staining 5 min. to overnight
Storage: Some Components Require Refrigerator & Freezer Storage. CLICK HERE For Experiment #953 Components and Requirements.
Cat. #5066 Personal PCR LabStation™ PERFECT PARTNER
Includes: one EdvoCycler™ Jr. (Cat. #540), two M12 Complete™ Packages (Cat. #502-504 ), one QuadraSource™ Power Supply (Cat. #5010-Q ), two 5 - 50 µL Variable Micropipettes (Cat. #590), one TruBlu™ 2 Transilluminator (Cat. #557), and one 3.0 L Water Bath (Cat. #539).
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