Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0
DETERMINATION OF INORGANIC ANIONS BY ION CHROMATOGRAPHY
Revision 2.1 | August 1993
John D. Pfaff Inorganic Chemistry Branch Chemistry Research Division
ENVIRONMENTAL MONITORING SYSTEMS LABORATORY OFFICE OF RESEARCH AND DEVELOPMENT U.S. ENVIRONMENTAL PROTECTION AGENCY CINCINNATI, OHIO 45268
WITH NOTES FROM
The Technical Team at Inorganic Ventures
All copyright held in respect of the EPA’s Method 300.0/300.1 remains solely with the U.S. Environmental Protection Agency. Notations added herein to the EPA Method 300.0/300.1 serve solely as commentary prepared by the staff at Inorganic Ventures and do not represent the opinion of the method authors, including but not limited to the Environmental Monitory Systems Laboratory, the Office of Research and Development or the U.S. Environmental Protection Agency generally. Furthermore, Inorganic Ventures does in no way claim any rights in or to the content of the EPA’s Method 300.0/300.1 by adding notations therein.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
Your method detection limit is going to depend heavily on your
Having trouble with your IC method? Check out IV's website for technical resources or reach out to us directly to speak with a Technical Support Chemist! samples and your sample preparation steps. You will need to use Laboratory Fortified Blanks (LFBs), Laboratory Fortified Sample Matrices (LFMs), and Laboratory Reagent Blanks (LRBs). These terms are defined in section 3.
This table is a great reference to test the linearity of your calibration at high concentrations. During method development try preparing test solutions where concentrations vary widely to test how your % recovery is impacted as peaks broaden at high concentrations. You may see poor results when analytes at low concentrations are neighboring analytes at high concentrations.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
Running single analyte standards is the gold standard to determine retention times. However, fortifying your sample with your analytes of interest can help determine if the peaks in the sample are truly your analytes of interest or separate unknown analytes. There are several commercially available columns that will allow for analysis of both Part A & B analytes. We have had great success using the AS22 column from Thermo or the Metrosep A Supp 5 column from Metrohm. Section 3.11 was updated in EPA 300.1 to a certified reference material and removed the sourcing specificity in this version.
You should expect to make some changes to this method as you begin to run samples. IC advancements have made analysis easier since this method was orignially published. Make sure to note any changes in your SOPs if you are referencing this method on your ISO: 17025 scope.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
This water dip can be eliminated by preparing your samples and standards in your selected eluent.
Fluoride interferences can be a large issue for analysts. Make sure to run single element standards of fluoride and some of the low molecular weight organics to define accurate retention times. These interferences can be spiked into your
LFM to confirm their presence.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
Make sure to check the cleanliness of your sample bottles. This is especially critical if you change vendors.
IV CRMs are stable for up to 5 years when stored in our Transpiration Control Technology (TCT) packaging. Once opened, our standards are good to use for 12 months!
1000 mg/L stock standard solutions are available as ISO:17034 Certified Reference Materials from Inorganic Ventures as stock products. We have a range of eluent
concentrates available also.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
More information on MDLs can be found here: Code of Federal Regulations, Title 40.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
MDLs can be difficult to assign accurately. You will have to look into the noise of your instrument signal and overlaying replicate data may help determine actual peaks from baseline noise. Feel free to reach out to info@inorganicventures.com for Technical Support.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
You can gain a higher confidence in your check solutions by using a second- source standard compared to the standards used to construct your calibration curve.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
Inorganic Ventures offers high purity standards for Bromate, Chlorate, and Chlorite.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
Once you have validated your method, ask about our custom CRM products. We can provide ready-to-use or concentrated blends containing your selected analytes at
Injection volumes have lowered since this method was written. EPA 300.1 lists injection volumes closer to modern
concentrations dictated by your method.
standard operating settings.
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
EPA Method 300.0 Determination of Inorganic Anions by Ion Chromatography
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