Lipidomic Standards
Pharma
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UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820
UltimateSPLASH™ ONE was created to address the need for a comprehensive set of internal standards and greatly expands the number of chain length and unsaturation variants within a given class, which may be used to control for differences in ionization and fragmentation efficiency. These internal standards are intentionally designed to appear in the spectral gaps between naturally occurring lipid species, and their relative abundances have been varied to reflect the patterns found in nature. Additionally, UltimateSPLASH™ incorporates these features into more lipid classes than ever before; PI, PS, PG, LPI, LPS, and LPG are now included for even broader lipidomics applications. The resulting final mixture contains a total of 69 unique and highly pure lipids across 15 lipid classes, which have been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics-workflows.
Lipids comprise an amazingly complex and diverse set of molecules that are involved in numerous biological processes including energy storage/release, signaling, growth, disease, and death. Over the last 20 years, use of soft ionization techniques in conjunction with single and multistage mass spectrometry both with and without prior separations have enabled unprecedented levels of sensitivity, speed, and specificity for identification, structure determination, and quantitation of these highly diverse ensemble of molecules. However, one of the primary challenges that remained was the development of a comprehensive set of internal standards which would allow accurate quantitation of numerous individual molecular species.
Target conc (µg/mL)
Exact mass
Chemical formula
Compound name
(M-H) -
(M+H) +
(M-NH4) - (M+NH4) + (M-Na) -
(M+Na) + (M+AcO) -
14:0-13:0-14:0 TG-d5
713.6582 C 44 H 79 D 5 O 6 739.6738 C 46 H 81 D 5 O 6 767.7051 C 48 H 85 D 5 O 6 795.7364 C 50 H 89 D 5 O 6 823.7677 C 52 H 93 D 5 O 6 849.7834 C 54 H 95 D 5 O 6 875.799 C 56 H 97 D 5 O 6 901.8147 C 58 H 99 D 5 O 6 929.846 C 60 H 103 D 5 O 6
25
731.692
14:0-15:1-14:0 TG-d5
50
757.7077
14:0-17:1-14:0 TG-d5
75
785.739
16:0-15:1-16:0 TG-d5
100
813.7703
16:0-17:1-16:0 TG-d5
125
841.8016
16:0-19:2-16:0 TG-d5
100
867.8172
18:1-17:1-18:1 TG-d5
75
893.8329
18:1-19:2-18:1 TG-d5
50
919.8485
18:1-21:2-18:1 TG-d5
25
947.8798
14:1 cholesteryl-d7 ester 601.5815 C 41 H 63 D 7 O 2 16:1 cholesteryl-d7 ester 629.6128 C 43 H 67 D 7 O 2 18:1 cholesteryl-d7 ester 657.6441 C 45 H 71 D 7 O 2 20:3 cholesteryl-d7 ester 681.6441 C 47 H 71 D 7 O 2 22:4 cholesteryl-d7 ester 707.6598 C 49 H 73 D 7 O 2
25
619.6153
624.5707
50
647.6466
652.602
75
675.6779
680.6333
50
699.6779
704.6333
25
725.6936 725.6936 730.649
C16:1 Ceramide-d7
542.5404 C 34 H 58 D 7 NO 3 570.5717 C 36 H 62 D 7 NO 3 598.603 C 38 H 66 D 7 NO 3 626.6343 C 40 H 70 D 7 NO 3 654.6656 C 42 H 74 D 7 NO 3
75
541.5331 543.5477
565.5296 601.5542
UltimateSPLASH™ ONE design strategy
C18:1 Ceramide-d7
50
569.5644 571.579
593.5609 629.5855
C20:1 Ceramide-d7
25
597.5957 599.6103
621.5922 657.6168
35:1
C22:1 Ceramide-d7
50
625.627 627.6416
649.6235 685.6481
17:0
PE 31:1 d5
C24:1 Ceramide-d7
75
653.6583 655.6729
677.6548 713.6794
O D DD D
O P
16:1 SM (d18:1/16:1)-d9 709.6084 C 39 H 68 D 9 N 2 O 6 P 75 18:1 SM (d18:1/18:1)-d9 737.6397 C 41 H 72 D 9 N 2 O 6 P 50 20:1 SM (d18:1/20:1)-d9 765.671 C 43 H 76 D 9 N 2 O 6 P 25 22:1 SM (d18:1/22:1)-d9 793.7023 C 45 H 80 D 9 N 2 O 6 P 50 24:1 SM (d18:1/24:1)-d9 821.7336 C 47 H 84 D 9 N 2 O 6 P 75
710.6157
768.6223
O D O O
O
33:1
37:3
HO
738.647
796.6536
NH 2
O
766.6783
824.6849
14:1
794.7096
852.7162
31:1
39.4
822.7409
880.7475
15:0 19:0 17:0
17:0-14:1 PC-d5
722.5622 C 39 H 71 D 5 NO 8 P 50 750.5935 C 41 H 75 D 5 NO 8 P 100 778.6248 C 43 H 79 D 5 NO 8 P 150 802.6248 C 45 H 79 D 5 NO 8 P 100 828.6405 C 47 H 81 D 5 NO 8 P 50 680.5153 C 36 H 65 D 5 NO 8 P 25 708.5466 C 38 H 69 D 5 NO 8 P 50 736.5779 C 40 H 73 D 5 NO 8 P 75 760.5779 C 42 H 73 D 5 NO 8 P 50 786.5935 C 44 H 75 D 5 NO 8 P 25 733.4918 C 37 H 65 D 5 NaO 10 P 25
723.5695
781.5761
17:0-16:1 PC-d5
751.6008
809.6074
14 16 18 20
30 32 34 36 38 40
17:0-18:1 PC-d5
779.6321
837.6387
17:0-20:3 PC-d5
803.6321
861.6387
Figure 1: Simulated RP separation of total acyl chain carbon for PE and LPE
17:0-22:4 PC-d5
829.6478
887.6543
Each unit of UltimateSPLASH™ ONE contains 69 high-purity, deuterium-labeled internal standards in a single vial of 1.2 mL of 1:1 DCM:MeOH. Each component has been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics techniques.
17:0-14:1 PE-d5
679.508 681.5226
In addition to our easy to use mixture of all 69 internal standards in one vial, UltimateSPLASH™ internal standards are available in class specific mixtures, as well as individual internal standards. Details on ordering individual internal standards can be found on our website, along with the 15 separate class mixtures.
17:0-16:1 PE-d5
707.5393 709.5539
17:0-18:1 PE-d5
735.5706 737.5852
17:0-20:3 PE-d5
759.5706 761.5852
17:0-22:4 PE-d5
785.5863 787.6008
17:0-14:1 PG-d5
734.4991
751.5256 710.5026
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MSI SPLASH TM Product number 330841
UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820
New product
As the role of lipids in neurodegenerative diseases such as Parkinson’s Disease is increasingly acknowledged, it has become essential to consider what lipids may have a hand in neuropathology. MSI SPLASH is made specifically for Mass Spec Imaging of neurological tissue. The mixture of thirteen internal standards is designed to be applied to brain sections using a sprayer, similarly to the application of matrix for MALDI. MSI SPLASH allows not only for quantitation of lipids within the brain, but also localization of those lipids to provide more detailed information for answering biological questions. This mixture was designed in collaboration with Michiel Vandenbosch, Shadrack Mutuku, Shane Ellis & Kim Ekroos. Toward Omics-Scale Quantitative Mass Spectrometry Imaging of Lipids in Brain Tissue Using a Multiclass Internal Standard Mixture. Michiel Vandenbosch, Shadrack M. Mutuku, Maria José Q. Mantas, Nathan H. Patterson, Tucker Hallmark, Marc Claesen, Ron M. A. Heeren, Nathan G. Hatcher, Nico Verbeeck, Kim Ekroos, and Shane R. Ellis. Analytical Chemistry 2023 95 (51), 18719-18730 DOI: 10.1021/acs.analchem.3c02724
Target conc (µg/mL)
Exact mass
Chemical formula
Compound name
(M-H) -
(M+H) +
(M-NH4) - (M+NH4) + (M-Na) -
(M+Na) + (M+AcO) -
17:0-16:1 PG-d5
761.5231 C 39 H 69 D 5 NaO 10 P 789.5544 C 41 H 73 D 5 NaO 10 P 813.5544 C 43 H 73 D 5 NaO 10 P 839.5701 C 45 H 75 D 5 NaO 10 P 746.4871 C 37 H 64 D 5 NaO 10 P 774.5184 C 39 H 68 D 5 NaO 10 P 802.5497 C 41 H 72 D 5 NaO 10 P 826.5497 C 43 H 72 D 5 NaO 10 P 852.5653 C 45 H 74 D 5 NaO 10 P 557.5068 C 34 H 59 D 5 O 5 585.5381 C 36 H 63 D 5 O 5 613.5694 C 38 H 67 D 5 O 5 637.5694 C 40 H 67 D 5 O 5 663.585 C 42 H 69 D 5 O 5 816.5525 C 40 H 73 D 5 NO 13 P 844.5838 C 42 H 77 D 5 NO 13 P 872.6151 C 44 H 81 D 5 NO 13 P 896.6151 C 46 H 81 D 5 NO 13 P 922.6307 C 48 H 83 D 5 NO 13 P 580.3384 C 24 H 45 D 5 NO 12 P 608.3697 C 26 H 49 D 5 NO 12 P 636.401 C 28 H 53 D 5 NO 12 P
50
762.5304
779.5569 738.5339
17:0-18:1 PG-d5
75
790.5617
807.5882 766.5652
17:0-20:3 PG-d5
50
814.5617
831.5882 790.5652
17:0-22:4 PG-d5
25
840.5773
857.6039 816.5808
17:0-14:1 PS-d5
25
745.4798 747.4943
723.4978
17:0-16:1 PS-d5
50
773.5111 775.5256
751.5291
17:0-18:1 PS-d5
75
801.5424 803.5569
779.5604
17:0-20:3 PS-d5
50
825.5424 827.5569
803.5604
Compound name
Exact mass Chemical formula Target conc (µM)
Target conc (µg/mL)
17:0-22:4 PS-d5
25
851.558 853.5726
829.5761
15:0-18:1 (d7) PA
689.499
C 39 H 69 D 5 NaO 10 P C 41 H 73 D 5 NaO 10 P C 43 H 73 DD 5 NaO 10 P C 45 H 75 DD 5 NaO 10 P C 37 H 64 D 5 NNaO 10 P C 39 H 68 D 5 NNaO 10 P C 41 H 72 D 5 NNaO 10 P C 43 H 72 D 5 NNaO 10 P C 45 H 74 D 5 NNaO 10 P
159.44
0.11
17:0-14:1 DG-d5
25
575.5406
580.496
17:0-16:1 DG-d5
50
603.5719
608.5273
15:0-18:1 (d7) PE
710.559
140.64
0.1
17:0-18:1 DG-d5
75
631.6032
636.5586
15:0-18:1 (d7) PG
763.536
64.14
0.049
17:0-20:3 DG-d5
50
655.6032
660.5586
15:0-18:1 (d7) PI
846.596
27.15
0.023
17:0-22:4 DG-d5
25
681.6188
686.5742
17:0-16:1 PS-d5
774.52
135.48
0.105
17:0-14:1 PI-d5
25
798.5186
17:0 Lyso PE-d5
472.33
6.35
0.003
17:0-16:1 PI-d5
50
826.5499
C12 Mono-sulfo galactosyl(beta) ceramide (d18:1/12:0)
740.486
25.64
0.019
17:0-18:1 PI-d5
75
854.5812
C15 Lactosyl(beta) ceramide-d7 (d18:1/15:0)
854.65
15.2
0.013
17:0-20:3 PI-d5
50
878.5812
C18 Ceramide-d7 (d18:1/18:0)
572.587
19.2
0.011
17:0-22:4 PI-d5
25
904.5969 940.6645
C17 Glucosyl(beta) Ceramide (d18:1/18:0)
713.581
C 34 H 59 D 5 O 5 C 36 H 63 D 5 O 5 C 38 H 67 D 5 O 5 C 40 H 67 D 5 O 5
186.26
0.133
15:0 Lyso PI-d5
25
562.3046 598.3723
SM d18:1/18:1(d9)
737.64
42
0.031
17:0 Lyso PI-d5
50
590.3359 626.4036
17:0 Lyso PC-d5
514.38
5.83
0.003
19:0 Lyso PI-d5
25
618.3672 654.4349
15:0-18:1 (d7) PC
752.606
213.79
0.161
15:0 Lyso PS-d5
510.273 C 21 H 36 D 5 NNaO 9 P 25 538.3043 C 23 H 40 D 5 NNaO 9 P 50 566.3356 C 25 H 44 D 5 NNaO 9 P 25 497.2778 C 21 H 37 D 5 NNaO 9 P 25 525.3091 C 23 H 41 D 5 NNaO 9 P 50 553.3404 C 25 H 45 D 5 NNaO 9 P 25
509.2658
487.2838
17:0 Lyso PS-d5
537.2971
515.3151
19:0 Lyso PS-d5
565.3284
543.3464
15:0 Lyso PG-d5
474.2886
17:0 Lyso PG-d5
502.3199
19:0 Lyso PG-d5
530.3512
15:0 Lyso PC-d5
486.3482 C 23 H 43 D 5 NO 7 P 514.3795 C 25 H 47 D 5 NO 7 P 542.4108 C 27 H 51 D 5 NO 7 P 444.3013 C 20 H 37 D 5 NO 7 P 472.3326 C 22 H 41 D 5 NO 7 P 500.3639 C 24 H 45 D 5 NO 7 P
25
485.341 487.3555
545.3621
17:0 Lyso PC-d5
50
513.3723 515.3868
573.3934
19:0 Lyso PC-d5
25
541.4036 543.4181
601.4247
15:0 Lyso PE-d5
25
443.294 445.3086
17:0 Lyso PE-d5
50
471.3253 473.3399
19:0 Lyso PE-d5
25
499.3566 501.3712
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LightSPLASH TM LIPIDOMIX TM Mass Spec Standard Product number 330732
EquiSPLASH™ LIPIDOMIX TM Mass Spec Standard Product number 330731
EquiSPLASH TM is popular for its higher concentrations of internal standard for each lipid class, allowing users to apply to multiple sample types (tissues, cell extracts, plasma). Each vial contains 1mL of internal standard mixture in methanol solution, 13 lipid classes supplied at 100μg/mL each. Concentrations are verified for use as a quantitative standard. This standard is also ideal for use in method development and system verification experiments to determine linearity and sensitivity prior to analyzing samples.
This mixture is a complement to EquiSPLASH TM ; it contains the same lipids as EquiSPLASH TM but without deuterium labels. If you need a primary lipid standard mixture for your assay development, look no further!
Compound name
Exact mass
Chemical formula Target conc (µg/mL)
15:0-18:1 PC
745.56
C 41 H 80 NO 8 P C 26 H 52 NO 7 P C 38 H 74 NO 8 P C 23 H 46 NO 7 P C 39 H 74 NaO 10 P
100
18:1 Lyso PC
521.348
100
Compound name
Exact mass
Chemical formula Target conc (µM)
Target conc (µg/mL)
15:0-18:1 PE
703.52
100
15:0-18:1(d7) PC
752.61
C 41 H 73 D 7 NO 8 P C 26 H 45 D 7 NO 7 P C 38 H 67 D 7 NO 8 P C 23 H 39 D 7 NO 7 P
133
100
18:1 Lyso PE
479.301
100
18:1(d7) Lyso PC
528.39
141
100
15:0-18:1 PG
756.49
100
15:0-18:1(d7) PE
710.56
129
100
15:0-18:1 PI
839.55
C 42 H 82 NO 13 P
100
18:1(d7) Lyso PE
486.35
131
100
15:0-18:1 PS
769.49
C 39 H 73 NNaO 10 P
100
15:0-18:1(d7) PG (Na Salt)
763.54
C 39 H 67 D 7 NaO 10 P C 42 H 75 D 7 NO 13 P C 39 H 66 D 7 NNaO 10 P
118
100
15:0-18:1-15:0 TG
804.72
C 51 H 96 O 6 C 36 H 68 O 5 C 21 H 40 O 4 C 45 H 78 O 2
100
15:0-18:1(d7) PI (NH4 Salt)
846.6
189
100
15:0-18:1 DG
580.51
100
15:0-18:1(d7) PS (Na Salt)
776.53
205
100
18:1 MG
356.29
100
15:0-18:1(d7)-15:0 TG
811.77
C 51 H 89 D 7 O 6 C 36 H 61 D 7 O 5 C 21 H 33 D 7 O 4 C 45 H 71 D 7 O 2
152
100
18:1 Chol Ester
650.6
100
15:0-18:1(d7) DG
587.55
275
100
d18:1-18:1 SM
728.583
C 41 H 81 N 2 O 6 P
100
18:1(d7) MG
363.34
170
100
C15 Ceramide (d18:1/15:0)
523.5
C 33 H 65 NO 3
100
18:1(d7) Chol Ester
657.64
123
100
d18:1-18:1(d9) SM
737.64
C 41 H 72 D 9 N 2 O 6 P
135
100
C15 Ceramide-d7 (d18:1-d7/15:0)
530.54
C 33 H 58 D 7 NO 3
188
100
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Oxysterol SPLASH TM Product number 330700
SPLAS H TM LIPIDOMIX TM Mass Spec Standard Product number 330707
Best seller
Oxysterols are intermediates in the biosynthesis of both bile acids and steroids, but some may also have their own biological activities in vivo. Conditions such as Alzheimer’s Disease, Multiple Sclerosis, and various cancers may be connected to altered oxysterol levels as well. OxysterolSPLASH TM contains 13 deuterated oxysterols at equal concentrations, making the mixture a useful tool for lipidomic analysis of these compounds.
The original SPLASH TM internal standard mixture has been a best-seller since it was first developed in 2016. It contains one deuterium labeled internal standard for each of 14 lipid classes at ratios relative to human plasma lipids. Each vial contains 1mL of internal standard mixture in methanol solution with a suggested sample spiking ratio of 10μL SPLASH TM to 10μL human plasma, allowing for up to 100 samples to be analyzed with one vial. Concentrations are verified for use as a quantitative standard.
Compound name
Exact mass
Chemical formula Target conc (µM)
Target conc (µg/mL)
Compound name
Exact mass
Chemical formula Target conc (µg/mL)
15:0-18:1(d7) PC
752.61
C 41 H 73 D 7 NO 8 P C 38 H 67 D 7 NO 8 P
160
213
24(R/S)-hydroxycholesterol-d7
409.394
C 27 H 39 D 7 O 2 C 27 H 40 D 6 O 2 C 27 H 40 D 6 O 2 C 27 H 39 O 2 D 7 C 27 H 39 O 2 D 7 C 27 H 37 O 2 D 7 C 27 H 37 D 7 O 2 C 27 H 40 D 6 O 3 C 27 H 40 D 6 O 3 C 27 H 39 D 3 O 4 C 27 H 39 O 2 D 7 C 27 H 39 O 2 D 7 C 27 H 41 D 7 O 3
150
5
8
15:0-18:1(d7) PE
710.56
25-hydroxycholesterol-d6
408.387
150
C 39 H 66 D 7 NNaO 10 P C 39 H 67 D 7 NaO 10 P
5
5
15:0-18:1(d7) PS (Na Salt)
776.53
27-hydroxycholesterol-d6
408.387
150
30
38
15:0-18:1(d7) PG (Na Salt)
763.54
7α-hydroxycholesterol-d7
409.394
150
C 42 H 75 D 7 NO 13 P C 36 H 61 D 7 NaO 8 P C 26 H 45 D 7 NO 7 P C 23 H 39 D 7 NO 7 P
10
11
15:0-18:1(d7) PI (NH4 Salt)
846.6
7ß-hydroxycholesterol-d7
409.394
150
7
11
15:0-18:1(d7) PA (Na Salt)
689.5
7-ketocholesterol-d7
407.378
150
25
48
18:1(d7) Lyso PC
528.39
7α-hydroxycholestenone-d7
407.378
150
5
11
18:1(d7) Lyso PE
486.35
7α,25-dihydroxycholesterol-d6
424.382
150
C 45 H 71 D 7 O 2 C 21 H 33 D 7 O 4 C 36 H 61 D 7 O 5 C 51 H 89 D 7 O 6
350
541
18:1(d7) Chol Ester
657.64
7α,27-dihydroxycholesterol-d6
424.382
150
2
6
18:1(d7) MAG
363.34
7α-Hydroxy-3-oxocholest-4-enoic acid-d3
433.33
150
10
16
15:0-18:1(d7) DAG
587.55
55
71
15:0-18:1(d7)-15:0 TAG
811.77
4ß-hydroxycholesterol-d7
409.394
150
C 41 H 72 D 9 N 2 O 6 P
30
42
d18:1-18:1(d9) SM
737.64
22(R)-hydroxycholesterol-d7
409.394
150
C 27 H 39 OD 7
100
248
Cholesterol (d7)
393.4
5α,6ß-dihydroxycholestanol-d7
427.404
150
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Light Bile Acid SPLASH TM Product number 330738 This mixture is a complement to Bile Acid SPLASH TM . It contains the same compounds as Bile Acid SPLASH TM but without deuterium labels, making this the ideal primary standard mixture for bile acid analysis.
Bile Acid SPLASH TM Product number 330736
Bile acids have long been known to play important functions in digestion and absorption of lipids. More recently, bile acids have been recognized as important signalling molecules with roles in microbiome homeostasis and metabolic disease. Dysregulation of bile acids can be indicative of various pathologies, making them promising targets of disease research. We created Bile Acid SPLASH TM so researchers can measure a wide array of bile acids in plasma using one pre-mixed internal standard mixture.
Compound name
Exact mass
Chemical formula Target conc (µM)
Cholic acid
408.29
C 24 H 40 O 5
1150
Compound name
Exact mass
Chemical formula
Target conc (µM)
Target conc (µg/mL)
Taurocholic acid, sodium salt
537.27
C 26 H 44 NNaO 7 S
1000
Cholic acid-d4
412.31
C 24 H 36 D 4 O 5
1150
0.474
Glycocholic acid
465.31
C 26 H 43 NO 6
1150
Taurocholic acid-d4, sodium salt
541.3
C 26 H 40 D 4 NNaO 7 S
1000
0.542
Deoxycholic acid
392.29
C 24 H 40 O 4
1650
Glycocholic-d4 acid
469.33
C 26 H 39 D 4 NO 6
1150
0.571
Taurodeoxycholic acid, sodium salt
521.28
C 26 H 44 NNaO 6 S
1000
Deoxycholic acid-d4
396.32
C 24 H 36 D 4 O 4
1650
0.654
Glycodeoxycholic acid
449.31
C 26 H 43 NO 5
1275
Taurodeoxycholic acid-d4, sodium salt
525.3
C 26 H 40 D 4 NNaO 6 S
1000
0.526
Chenodeoxycholic acid
392.29
C 24 H 40 O 4
1150
Glycodeoxycholic acid-d4
453.34
C 26 H 39 D 4 NO 5
1275
0.578
Taurochenodeoxycholic acid, sodium salt
521.28
C 26 H 44 NNaO 6 S
1000
Chenodeoxycholic acid-d4
396.32
C 24 H 36 D 4 O 4
1150
0.425
Glycochenodeoxycholic acid
449.31
C 26 H 43 NO 5
3800
Taurochenodeoxycholic acid-d4, sodium salt
525.3
C 26 H 40 D 4 NNaO 6 s
1000
0.526
Tauroursodeoxycholic acid, sodium salt
521.28
C 26 H 44 NNaO 6 S
500
Glycochenodeoxycholic-d4 acid
453.34
C 26 H 39 D 4 NO 5
3800
1.724
Glycoursodeoxycholic acid
449.31
C 26 H 43 NO 5 C 24 H 40 O 5 C 24 H 40 O 3
600
Tauroursodeoxycholic acid-d4, sodium salt
525.3
C 26 H 40 D 4 NNaO 6 S
500
0.263
3β,5α,6β-trihydroxycholanoic acid
408.29
250
Glycoursodeoxycholic acid-d4
453.34
C 26 H 39 D 4 NO 5 C 24 H 36 D 4 O 5 C 24 H 36 D 4 O 3
600
0.272
Lithocholic acid
376.3
250
3β,5α,6β-trihydroxycholanoic acid-d4
412.31
250
0.103
Taurolithocholic acid, sodium salt
505.28
C 26 H 44 NNaO 5 S
250
lithocholic acid-d4
380.32
250
0.095
Glycolithocholic acid
433.32
C 26 H 43 NO 4 C 24 H 40 O 4 C 24 H 38 O 3
250
Taurolithocholic acid-d4, sodium salt
509.31
C 26 H 40 D 4 NNaO 5 S
250
0.127
Ursodeoxycholic acid
392.29
300
Glycolithocholic-acid-d4
437.34
C 26 H 39 D 4 NO 4 C 24 H 36 D 4 O 4 C 24 H 34 D 4 O 3
250
0.109
Cholenic acid
374.28
250
Ursodeoxycholic acid-d4
396.32
300
0.119
Cholenic acid-d4
378.31
250
0.095
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Sphingo SPLASH TM I Product number 330734
Sphingolipids are increasingly recognized as a fascinating class of lipids involved in a variety of molecular functions from membrane composition to cellular signaling. As such, it is vital that sphingolipids can be accurately quantitated- that is why we developed SphingoSPLASH TM I . This mix is composed of equimolar concentrations of lipids representative of the sphingolipid class.
Compound name
Exact mass
Chemical formula
Target conc (µM)
Target conc (µg/mL)
C15 Ceramide-d7 (d18:1-D7/15:0)
530.54
C 33 H 58 D 7 NO 3 C 39 H 68 D 7 NO 8 C 37 H 64 D 7 NO 8 C 45 H 78 D 7 NO 13 C 31 H 56 D 7 NO 3 C 38 H 68 D 9 N 2 O 6 P
5.31
10
C15 Glucosyl(β) Ceramide-D7 (d18:1-D7/15:0)
692.59
6.93
10
C13 Galactosyl(β) Ceramide-D7 (d18:1-D7/13:0)
664.56
6.65
10
C15 Lactosyl(β) Ceramide-D7 (d18:1-D7/15:0)
854.65
8.55
10
C13 Dihydroceramide-d7 (d18:0-D7/13:0)
504.52
5.05
10
15:0 SM (d18:1/15:0)-D9
697.61
6.98
10
Sphingo SPLASH TM II Product number 330735
Carnitine SPLASH TM Product number 330379
Sphingosine and sphinganine serve as precursors to potent signaling molecules, and despite differing in only one double bond, the compounds have functionally distinct roles. Sphingadiene and the sphinganine precursor 3-ketosphinganine also have unique cellular activities. SphingoSPLASH TM II allows researchers to target these compounds for quantitative analysis.
Acylcarnitines transport fatty acids into mitochondria for β-oxidation, making them essential for the regulation of carbohydrate and lipid metabolism within a cell. A variety of health conditions are associated with dysregulated acylcarnitine levels, such as mitochondrial dysfunction, sepsis, cancer, and autoimmune disorders. Carnitine SPLASH TM contains deuterated forms of L-carnitine and 13 acylcarnitines for analysis of a wide array of sample types.
Compound name
Exact mass
Chemical formula
Target conc (µM)
Target conc (µg/mL)
Sphinganine D7
308.342
C 18 H 32 D 7 NO 2 C 18 H 30 D 7 NO 2 C 18 H 32 D 7 NO 2 C 18 H 31 D 7 ClNO 2
10
3.09
Sphingosine D7
306.326
10
3.07
Sphingadiene-d7
342.3
10
3.43
Compound name
Exact mass
Chemical formula
Target conc (µg/mL)
3-ketosphinganine-d7
304.31
10
3.05
L-carnitine-d9
170.16
C 7 H 6 D 9 NO 3 C 9 H 8 D 9 NO 4
10
Sphingo SPLASH TM III Product number 330735
C2:0 L-Carnitine-d9
212.17
10
C3:0 L-Carnitine-d9
262.16
C 10 H 11 D 9 ClNO 4 C 11 H 12 D 9 NO 4 C 12 H 14 D 9 NO 4 C 13 H 16 D 9 NO 4 C 15 H 20 D 9 NO 4 C 17 H 25 D 9 ClNO 4 C 19 H 29 D 9 ClNO 4 C 21 H 32 D 9 NO 4 C 23 H 36 D 9 NO 4 C 25 H 41 D 9 ClNO 4 C 25 H 38 D 9 NO 4 C 25 H 36 D 9 NO 4
10
C4:0 L-Carnitine-d9
240.2
10
C5:0 L-Carnitine-d9
254.22
10
While SphingoSPLASH TM I focuses on modified ceramides, SphingoSPLASH TM III targets modified sphingosine. This mix includes sugar-conjugated sphingosines, lyso-sulfatide, and lyso-sphingomyelin, making this a highly useful internal standard for those studying sphingosine metabolism.
C6:0 L-Carnitine-d9
268.23
10
C8:0 L-Carnitine-d9
296.27
10
C10:0 L-Carnitine-d9
360.27
10
Compound name
Exact mass
Chemical formula
Target conc (µM)
Target conc (µg/mL)
C12:0 L-Carnitine-d9
388.31
10
Lyso-sphingomyelin-d9
473.39
C 23 H 40 D 9 N 2 O 5 P C 24 H 40 D 7 NO 7 C 24 H 40 D 7 NO 7
10
4.74
C14:0 L-Carnitine-d9
380.36
10
Glucosyl(β) Sphingosine-d7
468.38
10
4.69
C16:0 L-Carnitine-d9
408.39
10
Galactosylsphingosine-d7
468.38
10
4.69
C18:0 L-Carnitine-d9
472.4
10
Lactosylsphingosine-d7
630.43
C 30 H 50 D 7 NO 12
10
6.31
C18:1 L-Carnitine-d9
434.41
10
3-O-sulfogalactosyl(beta) sphingosine-d7
565.36
C 24 H 43 D 7 N 2 O 10 S
10
5.66
C18:2 L-Carnitine-d9
432.39
10
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Mouse SPLASH TM Product number 330710
Ceramide LIPIDOMIX TM Product number 330712
Mouse SPLASH TM is similar to the original SPLASH TM , but with concentrations based on the lipid composition of murine samples.
Ceramides have significant clinical implications with regards to inflammation, immune response, and a variety of diseases. Ceramide LIPIDOMIX TM was developed to provide a clinically relevant ceramide mixture, and as suc1h it contains several key ceramides shown to be correlated to cardiovascular disease.
Compound name
Exact mass
Chemical formula Target conc (µM)
Target conc (µg/mL)
Compound name
Exact mass
Chemical formula
Target conc (µM)
Target conc (µg/mL)
15:0-18:1(d7) PC
752.61
C 41 H 73 D 7 NO 8 P C 38 H 67 D 7 NO 8 P
100
75.3
C16 Ceramide (d18:1/16:0)
537.512
C 34 H 67 NO 3 C 36 H 71 NO 3 C 42 H 83 NO 3 C 42 H 81 NO 3
30
16.1
15:0-18:1(d7) PE
710.56
7
5
C18 Ceramide (d18:1/18:0)
565.543
15
8.5
15:0-18:1(d7) PS (Na Salt)
776.53
C 39 H 66 D 7 NNaO 10 P C 39 H 67 D 7 NaO 10 P C 42 H 75 D 7 NO 13 P C 36 H 61 D 7 NaO 8 P C 26 H 45 D 7 NO 7 P C 23 H 39 D 7 NO 7 P
20
15.5
C24 Ceramide (d18:1/24:0)
649.637
75
48.8
15:0-18:1(d7) PG (Na Salt)
763.54
5
3.8
C24:1 Ceramide (d18:1/24:1(15Z))
647.622
37.5
24.3
15:0-18:1(d7) PI (NH4 Salt)
846.6
20
16.9
15:0-18:1(d7) PA (Na Salt)
689.5
10
6.9
1 European Heart Journal, Volume 37, Issue 25, 1 July 2016, Pages 1967–1976, https://doi.org/10.1093/eurheartj/ehw148
18:1(d7) Lyso PC
528.39
45
23.8
18:1(d7) Lyso PE
486.35
2
1
SPLASH TM II Product number 330709
18:1(d7) Chol Ester
657.64
C 45 H 71 D 7 O 2
250
164.5
C18(Plasm)-18:1(d9) PC
780.67
C 44 H 77 D 9 NO 7 P
20
15.6
15:0-18:1(d7) DAG
587.55
C 36 H 61 D 7 O 5 C 51 H 89 D 7 O 6
15
8.8
15:0-18:1(d7)-15:0 TAG
811.77
35
28.4
d18:1-18:1(d9) SM
737.64
C 41 H 72 D 9 N 2 O 6 P C 41 H 71 D 9 NO 7 P
20
14.8
SPLASH TM II is a variation on the original SPLASH TM designed for plasma lipidomics. Modifications include the addition of plasmalogens and the removal of cholesterol, PG, PA, and MAG.
C18(Plasm)-18:1(d9) PE
738.62
5
3.7
Compound name
Exact mass
Chemical formula
Target conc (µM)
Target conc (µg/mL)
15:0-18:1(d7) PC
752.61
C 41 H 73 D 7 NO 8 P C 38 H 67 D 7 NO 8 P
210
158.2
15:0-18:1(d7) PE
710.56
7
5
15:0-18:1(d7) PS (Na Salt)
776.53
C 39 H 66 D 7 NNaO 10 P C 42 H 75 D 7 NO 13 P C 26 H 45 D 7 NO 7 P
10
7.8
15:0-18:1(d7) PI (NH4 Salt)
846.6
10
8.5
18:1(d7) Lyso PC
528.39
45
23.80
18:1(d7) Lyso PE
486.35
C 23 H 39 D 7 NO 7 P
1
0.50
18:1(d7) Chol Ester
657.64
C 45 H 71 D 7 O 2
530
348.80
C18(Plasm)-18:1(d9) PC
780.67
C 44 H 77 D 9 NO 7 P
10
7.80
15:0-18:1(d7) DAG
587.55
C 36 H 61 D 7 O 5 C 51 H 89 D 7 O 6
20
11.80
15:0-18:1(d7)-15:0 TAG
811.77
70
56.90
d18:1-18:1(d9) SM
737.64
C 41 H 72 D 9 N 2 O 6 P C 41 H 71 D 9 NO 7 P
40
29.50
C18(Plasm)-18:1(d9) PE
738.62
0.1
0.07
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Frequently asked questions
Suggested extraction procedures For research use only. Not for use in diagnostic procedures.
How do I handle Avanti Mass Spec Standards properly? Your Avanti Mass Spec Standard should be stored in a -10°C to -25°C freezer until ready for use. It is designed to be a one-time use ampule, and we do not recommend extended storage after opening. Always make sure to warm bath sonicate the unopened ampoule for approximately 2 minutes prior to opening the ampoule. Lipids in solution may precipitate during shipping and storage conditions, and it may not be visible with a solution at extremely low concentrations such as this. Directly transfer from ampule to sample preparation glass vial for immediate use. General handling guidelines for lipids should be followed as outlined on our website. How do I order Avanti Mass Spec Standards? Please visit the Avanti website. Customers in the United States can order directly from Avanti, and customers outside the United States will be directed to our worldwide distribution partner for country specific ordering information and pricing. Can I use my Avanti Mass Spec Standard more than once? Avanti quantitative mass spectrometry standards are designed to be “one-time use” items. It is important to directly transfer from the ampule to the experiment or prepare as a dilution for immediate use. Avanti cannot guarantee product purity and subsequent performance if used outside these guidelines. How do I reference an Avanti Mass Spec Standard in my publication? Please list the full name and product number of the Avanti product in your materials and methods sections, along with “Avanti Research , Alabaster, AL” as the source. If you publish using an Avanti product we would love to hear about it! Please drop us an email and share your success stories.
How do I open the sealed glass ampule? Ampules are pre-scored for easy opening. With the ampule upright, hold the ampule in a gloved hand and firmly push the top section away from you with even pressure. The top should cleanly snap off, but proceed with care, as it will leave a sharp glass edge. Can I get a Custom Mass Spec Standard? Yes, we custom formulate mass spec standards using the lipids and solvents of your choice. We work with you to design your custom mass spec standard and can make recommendations if needed. Let us know how we can help by emailing lipidomics@avantilipids.com. Avanti has been making Custom Quantitative Mass Spec Standards for the Lipidomics community for over 15 years. We specialize in high-accuracy quantitative packaging of lipids for use as mass spec standards, robotic assisted packaging of solutions, accurate quantitation of lipid concentrations, and rigorous quality control testing. Who do I contact if I have additional questions? Please e-mail us at lipidomics@avantilipids.com if you have any additional questions. Custom Synthesis Avanti prides itself on being innovative by discovering new lipids for our customers. We are constantly adding to our extensive list of products for research. However, if you cannot find the perfect lipid to make your project a success, or you have a suggestion for a new product that is needed in the field, our synthesis team is ready to take on a new challenge. With over 150 years of combined lipid synthesis experience, our team can solve most synthesis problems and deliver custom lipids with Avanti’s signature quality. Please contact our synthesis team at customsynthesis@avantilipids.com to discuss the amazing things we can do for you.
Extraction Protocol for Plasma Using SPLASH TM or UltimateSPLASH™ ONE 1. Use 13 x 100 mm new glass screw capped tubes. Do not use washed tubes as you may extract detergent residue. 2. Add 990 μl water to 10 μl plasma, then let sit on ice for 10 minutes. 3. Add 2.0 mL methanol. 4. Add 0.9 mL dichloromethane. 5. Vortex. 6. A singe phase should appear. If there are two distinct phases, add 50 μl methanol and vortex. If solution is still not a single phase, repeat addition of 50 μl methanol and vortex. 7. Add 10 μl UltimateSPLASH™ ONE Internal Standard, vortex, and let mixture sit for 30 minutes at room temperature. 8. Add 1 mL water. 9. Add 0.9 mL dichloromethane.
4. For cells in suspension: centrifuge, discard saline, and add 1 mL water. Vortex and transfer to glass tube for extraction. Rest on ice for 10 minutes. Ensure final volume is 1 mL and adjust if necessary. 5. For adhered cells: wash cells with non-buffered saline. Add 1 mL water to lyse cells and scrap. Collect cell lysate and transfer to glass tube for extraction. Rest on ice for 10 minutes. Ensure final volume is 1 mL and adjust if necessary. 6. Add 2.0 mL methanol. Add 990 μl water to 10 μl plasma, then let sit on ice for 10 minutes. 7. Add 0.9 mL of dichloromethane. 8. Vortex. 9. Repeat steps 6-16 from Extraction Protocol for Plasma. Extraction Protocol for Cell Culture 1. Weigh tissue to be extracted. 50-100 mg is sufficient. Calculate water content. Expected values are as follows: a.Adipose 18% b. Brain 60% c. Bone 44% d. Heart, kidney, liver, lung, intestines, spleen, and stomach 65% e.Testes 18% 2. Add water to tissue so that total volume is 1 mL. Example: 100 mg of brain tissue corresponds to 60 μL water. Add 940 μL water. 3. Disperse tissue. a. Grind tissue frozen in liquid nitrogen using cold mortar and pestle. b. Blend using a homogenizer. 4. Sonicate for 30 seconds with 5 seconds bursts and 20 second rest time. Perform sonication steps on ice. 5. Add 2.0 mL of methanol. 6. Add 0.9 mL dichloromethane. 7. Vortex. 8. Repeat steps 6-16 from Extraction Protocol for Plasma.
10.Invert tube 10 times. DO NOT VORTEX. 11.Centrifuge at 1200 rpm for 10 minutes.
12.Collect lower layer and put into a new glass tube. 13.Add 2 mL dichloromethane to remains in extraction tube. 14.Mix, centrifuge, and collect lower layer. Add to first extract. 15.Evaporate solvent under a stream of nitrogen. 16.Re-suspend lipids in injection solvent. Extraction Protocol for Cell Culture 1. Use 13 x 100 mm new glass screw capped tubes. Do not use washed tubes as you may extract detergent residue. 2. Collect cells. 3. Wash cells with non-buffered saline to remove cell culture
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Other Products
Bile acids Bile acids are potent digestive surfactants which promote the absorption of lipids. They also represent the primary pathway for cholesterol catabolism and account for approximately 50% of the daily turnover of cholesterol 2 . However, bile acids are no longer limited to solely that. Bile acids have now been recognized as hormones involved in the regulation of various metabolic processes. Bile acids regulate not only their own synthesis and enterohepatic circulation, but also triglyceride, cholesterol, glucose and energy homeostasis. Current metabolomic and lipidomic efforts aim to provide new insights into the mechanism of bile acid signaling in the regulation of lipid metabolism and the maintenance of lipid homeostasis. 1,2 Avanti’s comprehensive line of pure bile acid primary standards and internal standards are the perfect complement to primary, secondary, and/or conjugated bile acid research in any clinical, toxicology, or research laboratory. The table is just a sample of the many bile acid standards that Avanti offers. Visit the website links below to discover the full lineup.
IsoPure Phospholipids Mixed-acyl glycerophospholipids are among the most abundant lipids in nature. Famously, 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) is the most abundant phospholipid in most eukaryotic cells and tissues. The molecular structure of POPC follows the textbook consensus that unsaturated fatty acyl chains occupy the sn-2 position of the glycerol backbone while saturated fatty acyl chains are relegated to the sn-1 position. As our analytical technologies advance, this conventional wisdom is being turned inside out with the isomer 1-oleoyl-2-palmitoyl phosphatidylcholine (OPPC) being identified at high abundance in samples ranging from plasma to tissue 1,2,3 . Adding to the complexity, both isomers are present in many instances in ratios ranging from 1:100 to 1:1. Resolving the puzzle of lipid isomers requires advanced analytical tools in conjunction with structurally defined reference materials. Until recently, commercially available synthetic lipids were also comprised of mixtures, as transacylation occurring in the round-bottom flask always led to 10-20% OPPC in any preparation of POPC. Moreover, the regiopurity of the product was difficult to define. Avanti has now introduced the IsoPure line wherein innovative synthetic procedures produce mixed-acyl glycerophospholipids with >99% regiopurity. These next-generation standards will be pivotal to isomer-resolved identification of glycerophospholipids in biological samples. 1 Ekroos K, Ejsing CS, Bahr U, Karas M, Simons K, Shevchenko A. Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation. J. Lipid Res. 2003, 44, 2181. PMID: 12923235 2 Zacek P, Bukowski M, Rosenberger TA, Picklo M. Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer. J. Lipid Res. 2016, 57, 2225. PMID: 27688258 3 Maccarone AT, Duldig J, Mitchell TW, Blanksby SJ, Duchoslav E, Campbell JL. Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry.J. Lipid Res. 2014, 55, 1668. PMID: 24939921
Compound name
Exact mass
Chemical formula
Cholic acid-d4
412.31
C 24 H 36 D 4 O 5 C 24 H 36 D 4 O 4 C 24 H 36 D 4 O 3 C 24 H 36 D 4 O 4 C 24 H 36 D 4 O 4 C 26 H 39 D 4 NO 5
Deoxycholic acid-d4
396.32
Lithocholic acid-d4
380.32
Chenodeoxycholic acid-(2,2,4,4-d4)
396.32
Ursodeoxycholic acid-d4
396.32
Glycodeoxycholic acid-d4
453.34
Taurodeoxycholic acid-d4, sodium salt
525.3
C 26 H 40 D 4 NNaO 6 S
IsoPure Phospholipids Now Available from Avanti https://avantilipids.com/product-category/phospholipids/isopure
Glycochenodeoxycholic acid-d4
453.34
C 26 H 39 D 4 NO 5
Taurochenodeoxycholic acid-d4, sodium salt
525.3
C 26 H 40 D 4 NNaO 6 S
Glycolithocholic acid-d4
437.34
C 26 H 39 D 4 NO 5 C 26 H 39 D 4 NO 5 C 26 H 39 D 4 NO 6
Glycoursodeoxycholic acid-d4
453.34
Glycocholic acid-d4
469.33
Taurocholic acid-d4, sodium salt
541.3
C 26 H 40 D 4 NNaO 7 S C 26 H 40 D 4 NNaO 6 S C 26 H 40 D 4 NNaO 5 S
Tauroursodeoxycholic acid-d4, sodium salt
525.3
Taurolithocholic acid-d4, sodium salt
509.31
1 Qi Y, Jiang C, Cheng J, Krausz KW, Li T, Ferrell JM, Gonzalez FJ, Chiang JY. Bile acid signaling in lipid metabolism: metabolomic and lipidomic analysis of lipid and bile acid markers linked to anti-obesity and anti-diabetes in mice. Biochim Biophys Acta. 2015 Jan;1851(1):19-29. PMID: 24796972 2 Staels B, Fonseca VA. Bile acids and metabolic regulation: mechanisms and clinical responses to bile acid sequestration. Diabetes Care. 2009 Nov;32 Suppl 2(Suppl 2):S237-45. PMID: 19875558 Bile Acid Primary Standards Now Available from Avanti: https://avantilipids.com/product-category/bile-acids Bile Acid Internal Standards Now Available from Avanti: https://avantilipids.com/product-category/lipidomics/bile-acid-standards
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@AvantiResearch
Avanti Research
lipidomics@avantilipids.com
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Non-warranty The information in this publication is believed to be accurate and is given in good faith, but no representation or warranty as to its completeness or accuracy is made. Suggestions for uses or applications are only opinions. Users are responsible for determining the suitability of these products for their own particular purpose. No representation or warranty, expressed or implied, is made with respect to information or products including, without limitation, warranties of merchantability, fitness for a particular purpose, non-infringement of any third-party patent or other intellectual property rights including, without limit, copyright, trademark and designs. Unless otherwise stated, any trademarks identified herein are trademarks of the Croda group of companies. Avanti Research is a trademark of Croda international Plc. Avanti Research is a Croda brand associated with Avanti Polar Lipids, LLC.
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