SPLASH Brochure

Light Bile Acid SPLASH TM Product number 330738 This mixture is a complement to Bile Acid SPLASH TM . It contains the same compounds as Bile Acid SPLASH TM but without deuterium labels, making this the ideal primary standard mixture for bile acid analysis.

Bile Acid SPLASH TM Product number 330736

Bile acids have long been known to play important functions in digestion and absorption of lipids. More recently, bile acids have been recognized as important signalling molecules with roles in microbiome homeostasis and metabolic disease. Dysregulation of bile acids can be indicative of various pathologies, making them promising targets of disease research. We created Bile Acid SPLASH TM so researchers can measure a wide array of bile acids in plasma using one pre-mixed internal standard mixture.

Compound name

Exact mass

Chemical formula Target conc (µM)

Cholic acid

408.29

C 24 H 40 O 5

1150

Compound name

Exact mass

Chemical formula

Target conc (µM)

Target conc (µg/mL)

Taurocholic acid, sodium salt

537.27

C 26 H 44 NNaO 7 S

1000

Cholic acid-d4

412.31

C 24 H 36 D 4 O 5

1150

0.474

Glycocholic acid

465.31

C 26 H 43 NO 6

1150

Taurocholic acid-d4, sodium salt

541.3

C 26 H 40 D 4 NNaO 7 S

1000

0.542

Deoxycholic acid

392.29

C 24 H 40 O 4

1650

Glycocholic-d4 acid

469.33

C 26 H 39 D 4 NO 6

1150

0.571

Taurodeoxycholic acid, sodium salt

521.28

C 26 H 44 NNaO 6 S

1000

Deoxycholic acid-d4

396.32

C 24 H 36 D 4 O 4

1650

0.654

Glycodeoxycholic acid

449.31

C 26 H 43 NO 5

1275

Taurodeoxycholic acid-d4, sodium salt

525.3

C 26 H 40 D 4 NNaO 6 S

1000

0.526

Chenodeoxycholic acid

392.29

C 24 H 40 O 4

1150

Glycodeoxycholic acid-d4

453.34

C 26 H 39 D 4 NO 5

1275

0.578

Taurochenodeoxycholic acid, sodium salt

521.28

C 26 H 44 NNaO 6 S

1000

Chenodeoxycholic acid-d4

396.32

C 24 H 36 D 4 O 4

1150

0.425

Glycochenodeoxycholic acid

449.31

C 26 H 43 NO 5

3800

Taurochenodeoxycholic acid-d4, sodium salt

525.3

C 26 H 40 D 4 NNaO 6 s

1000

0.526

Tauroursodeoxycholic acid, sodium salt

521.28

C 26 H 44 NNaO 6 S

500

Glycochenodeoxycholic-d4 acid

453.34

C 26 H 39 D 4 NO 5

3800

1.724

Glycoursodeoxycholic acid

449.31

C 26 H 43 NO 5 C 24 H 40 O 5 C 24 H 40 O 3

600

Tauroursodeoxycholic acid-d4, sodium salt

525.3

C 26 H 40 D 4 NNaO 6 S

500

0.263

3β,5α,6β-trihydroxycholanoic acid

408.29

250

Glycoursodeoxycholic acid-d4

453.34

C 26 H 39 D 4 NO 5 C 24 H 36 D 4 O 5 C 24 H 36 D 4 O 3

600

0.272

Lithocholic acid

376.3

250

3β,5α,6β-trihydroxycholanoic acid-d4

412.31

250

0.103

Taurolithocholic acid, sodium salt

505.28

C 26 H 44 NNaO 5 S

250

lithocholic acid-d4

380.32

250

0.095

Glycolithocholic acid

433.32

C 26 H 43 NO 4 C 24 H 40 O 4 C 24 H 38 O 3

250

Taurolithocholic acid-d4, sodium salt

509.31

C 26 H 40 D 4 NNaO 5 S

250

0.127

Ursodeoxycholic acid

392.29

300

Glycolithocholic-acid-d4

437.34

C 26 H 39 D 4 NO 4 C 24 H 36 D 4 O 4 C 24 H 34 D 4 O 3

250

0.109

Cholenic acid

374.28

250

Ursodeoxycholic acid-d4

396.32

300

0.119

Cholenic acid-d4

378.31

250

0.095

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