UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820
UltimateSPLASH™ ONE was created to address the need for a comprehensive set of internal standards and greatly expands the number of chain length and unsaturation variants within a given class, which may be used to control for differences in ionization and fragmentation efficiency. These internal standards are intentionally designed to appear in the spectral gaps between naturally occurring lipid species, and their relative abundances have been varied to reflect the patterns found in nature. Additionally, UltimateSPLASH™ incorporates these features into more lipid classes than ever before; PI, PS, PG, LPI, LPS, and LPG are now included for even broader lipidomics applications. The resulting final mixture contains a total of 69 unique and highly pure lipids across 15 lipid classes, which have been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics-workflows.
Lipids comprise an amazingly complex and diverse set of molecules that are involved in numerous biological processes including energy storage/release, signaling, growth, disease, and death. Over the last 20 years, use of soft ionization techniques in conjunction with single and multistage mass spectrometry both with and without prior separations have enabled unprecedented levels of sensitivity, speed, and specificity for identification, structure determination, and quantitation of these highly diverse ensemble of molecules. However, one of the primary challenges that remained was the development of a comprehensive set of internal standards which would allow accurate quantitation of numerous individual molecular species.
UltimateSPLASH™ ONE design strategy
35:1
17:0
PE 31:1 d5
O D DD D
O P
O D O O
O
33:1
37:3
HO
NH 2
O
14:1
31:1
39.4
15:0 19:0 17:0
14 16 18 20
30 32 34 36 38 40
Figure 1: Simulated RP separation of total acyl chain carbon for PE and LPE
Each unit of UltimateSPLASH™ ONE contains 69 high-purity, deuterium-labeled internal standards in a single vial of 1.2 mL of 1:1 DCM:MeOH. Each component has been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics techniques.
In addition to our easy to use mixture of all 69 internal standards in one vial, UltimateSPLASH™ internal standards are available in class specific mixtures, as well as individual internal standards. Details on ordering individual internal standards can be found on our website, along with the 15 separate class mixtures.
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