SPLASH Brochure

UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820

UltimateSPLASH™ ONE was created to address the need for a comprehensive set of internal standards and greatly expands the number of chain length and unsaturation variants within a given class, which may be used to control for differences in ionization and fragmentation efficiency. These internal standards are intentionally designed to appear in the spectral gaps between naturally occurring lipid species, and their relative abundances have been varied to reflect the patterns found in nature. Additionally, UltimateSPLASH™ incorporates these features into more lipid classes than ever before; PI, PS, PG, LPI, LPS, and LPG are now included for even broader lipidomics applications. The resulting final mixture contains a total of 69 unique and highly pure lipids across 15 lipid classes, which have been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics-workflows.

Lipids comprise an amazingly complex and diverse set of molecules that are involved in numerous biological processes including energy storage/release, signaling, growth, disease, and death. Over the last 20 years, use of soft ionization techniques in conjunction with single and multistage mass spectrometry both with and without prior separations have enabled unprecedented levels of sensitivity, speed, and specificity for identification, structure determination, and quantitation of these highly diverse ensemble of molecules. However, one of the primary challenges that remained was the development of a comprehensive set of internal standards which would allow accurate quantitation of numerous individual molecular species.

Target conc (µg/mL)

Exact mass

Chemical formula

Compound name

(M-H) -

(M+H) +

(M-NH4) - (M+NH4) + (M-Na) -

(M+Na) + (M+AcO) -

14:0-13:0-14:0 TG-d5

713.6582 C 44 H 79 D 5 O 6 739.6738 C 46 H 81 D 5 O 6 767.7051 C 48 H 85 D 5 O 6 795.7364 C 50 H 89 D 5 O 6 823.7677 C 52 H 93 D 5 O 6 849.7834 C 54 H 95 D 5 O 6 875.799 C 56 H 97 D 5 O 6 901.8147 C 58 H 99 D 5 O 6 929.846 C 60 H 103 D 5 O 6

25

731.692

14:0-15:1-14:0 TG-d5

50

757.7077

14:0-17:1-14:0 TG-d5

75

785.739

16:0-15:1-16:0 TG-d5

100

813.7703

16:0-17:1-16:0 TG-d5

125

841.8016

16:0-19:2-16:0 TG-d5

100

867.8172

18:1-17:1-18:1 TG-d5

75

893.8329

18:1-19:2-18:1 TG-d5

50

919.8485

18:1-21:2-18:1 TG-d5

25

947.8798

14:1 cholesteryl-d7 ester 601.5815 C 41 H 63 D 7 O 2 16:1 cholesteryl-d7 ester 629.6128 C 43 H 67 D 7 O 2 18:1 cholesteryl-d7 ester 657.6441 C 45 H 71 D 7 O 2 20:3 cholesteryl-d7 ester 681.6441 C 47 H 71 D 7 O 2 22:4 cholesteryl-d7 ester 707.6598 C 49 H 73 D 7 O 2

25

619.6153

624.5707

50

647.6466

652.602

75

675.6779

680.6333

50

699.6779

704.6333

25

725.6936 725.6936 730.649

C16:1 Ceramide-d7

542.5404 C 34 H 58 D 7 NO 3 570.5717 C 36 H 62 D 7 NO 3 598.603 C 38 H 66 D 7 NO 3 626.6343 C 40 H 70 D 7 NO 3 654.6656 C 42 H 74 D 7 NO 3

75

541.5331 543.5477

565.5296 601.5542

UltimateSPLASH™ ONE design strategy

C18:1 Ceramide-d7

50

569.5644 571.579

593.5609 629.5855

C20:1 Ceramide-d7

25

597.5957 599.6103

621.5922 657.6168

35:1

C22:1 Ceramide-d7

50

625.627 627.6416

649.6235 685.6481

17:0

PE 31:1 d5

C24:1 Ceramide-d7

75

653.6583 655.6729

677.6548 713.6794

O D DD D

O P

16:1 SM (d18:1/16:1)-d9 709.6084 C 39 H 68 D 9 N 2 O 6 P 75 18:1 SM (d18:1/18:1)-d9 737.6397 C 41 H 72 D 9 N 2 O 6 P 50 20:1 SM (d18:1/20:1)-d9 765.671 C 43 H 76 D 9 N 2 O 6 P 25 22:1 SM (d18:1/22:1)-d9 793.7023 C 45 H 80 D 9 N 2 O 6 P 50 24:1 SM (d18:1/24:1)-d9 821.7336 C 47 H 84 D 9 N 2 O 6 P 75

710.6157

768.6223

O D O O

O

33:1

37:3

HO

738.647

796.6536

NH 2

O

766.6783

824.6849

14:1

794.7096

852.7162

31:1

39.4

822.7409

880.7475

15:0 19:0 17:0

17:0-14:1 PC-d5

722.5622 C 39 H 71 D 5 NO 8 P 50 750.5935 C 41 H 75 D 5 NO 8 P 100 778.6248 C 43 H 79 D 5 NO 8 P 150 802.6248 C 45 H 79 D 5 NO 8 P 100 828.6405 C 47 H 81 D 5 NO 8 P 50 680.5153 C 36 H 65 D 5 NO 8 P 25 708.5466 C 38 H 69 D 5 NO 8 P 50 736.5779 C 40 H 73 D 5 NO 8 P 75 760.5779 C 42 H 73 D 5 NO 8 P 50 786.5935 C 44 H 75 D 5 NO 8 P 25 733.4918 C 37 H 65 D 5 NaO 10 P 25

723.5695

781.5761

17:0-16:1 PC-d5

751.6008

809.6074

14 16 18 20

30 32 34 36 38 40

17:0-18:1 PC-d5

779.6321

837.6387

17:0-20:3 PC-d5

803.6321

861.6387

Figure 1: Simulated RP separation of total acyl chain carbon for PE and LPE

17:0-22:4 PC-d5

829.6478

887.6543

Each unit of UltimateSPLASH™ ONE contains 69 high-purity, deuterium-labeled internal standards in a single vial of 1.2 mL of 1:1 DCM:MeOH. Each component has been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics techniques.

17:0-14:1 PE-d5

679.508 681.5226

In addition to our easy to use mixture of all 69 internal standards in one vial, UltimateSPLASH™ internal standards are available in class specific mixtures, as well as individual internal standards. Details on ordering individual internal standards can be found on our website, along with the 15 separate class mixtures.

17:0-16:1 PE-d5

707.5393 709.5539

17:0-18:1 PE-d5

735.5706 737.5852

17:0-20:3 PE-d5

759.5706 761.5852

17:0-22:4 PE-d5

785.5863 787.6008

17:0-14:1 PG-d5

734.4991

751.5256 710.5026

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