UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820
UltimateSPLASH™ ONE was created to address the need for a comprehensive set of internal standards and greatly expands the number of chain length and unsaturation variants within a given class, which may be used to control for differences in ionization and fragmentation efficiency. These internal standards are intentionally designed to appear in the spectral gaps between naturally occurring lipid species, and their relative abundances have been varied to reflect the patterns found in nature. Additionally, UltimateSPLASH™ incorporates these features into more lipid classes than ever before; PI, PS, PG, LPI, LPS, and LPG are now included for even broader lipidomics applications. The resulting final mixture contains a total of 69 unique and highly pure lipids across 15 lipid classes, which have been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics-workflows.
Lipids comprise an amazingly complex and diverse set of molecules that are involved in numerous biological processes including energy storage/release, signaling, growth, disease, and death. Over the last 20 years, use of soft ionization techniques in conjunction with single and multistage mass spectrometry both with and without prior separations have enabled unprecedented levels of sensitivity, speed, and specificity for identification, structure determination, and quantitation of these highly diverse ensemble of molecules. However, one of the primary challenges that remained was the development of a comprehensive set of internal standards which would allow accurate quantitation of numerous individual molecular species.
Target conc (µg/mL)
Exact mass
Chemical formula
Compound name
(M-H) -
(M+H) +
(M-NH4) - (M+NH4) + (M-Na) -
(M+Na) + (M+AcO) -
14:0-13:0-14:0 TG-d5
713.6582 C 44 H 79 D 5 O 6 739.6738 C 46 H 81 D 5 O 6 767.7051 C 48 H 85 D 5 O 6 795.7364 C 50 H 89 D 5 O 6 823.7677 C 52 H 93 D 5 O 6 849.7834 C 54 H 95 D 5 O 6 875.799 C 56 H 97 D 5 O 6 901.8147 C 58 H 99 D 5 O 6 929.846 C 60 H 103 D 5 O 6
25
731.692
14:0-15:1-14:0 TG-d5
50
757.7077
14:0-17:1-14:0 TG-d5
75
785.739
16:0-15:1-16:0 TG-d5
100
813.7703
16:0-17:1-16:0 TG-d5
125
841.8016
16:0-19:2-16:0 TG-d5
100
867.8172
18:1-17:1-18:1 TG-d5
75
893.8329
18:1-19:2-18:1 TG-d5
50
919.8485
18:1-21:2-18:1 TG-d5
25
947.8798
14:1 cholesteryl-d7 ester 601.5815 C 41 H 63 D 7 O 2 16:1 cholesteryl-d7 ester 629.6128 C 43 H 67 D 7 O 2 18:1 cholesteryl-d7 ester 657.6441 C 45 H 71 D 7 O 2 20:3 cholesteryl-d7 ester 681.6441 C 47 H 71 D 7 O 2 22:4 cholesteryl-d7 ester 707.6598 C 49 H 73 D 7 O 2
25
619.6153
624.5707
50
647.6466
652.602
75
675.6779
680.6333
50
699.6779
704.6333
25
725.6936 725.6936 730.649
C16:1 Ceramide-d7
542.5404 C 34 H 58 D 7 NO 3 570.5717 C 36 H 62 D 7 NO 3 598.603 C 38 H 66 D 7 NO 3 626.6343 C 40 H 70 D 7 NO 3 654.6656 C 42 H 74 D 7 NO 3
75
541.5331 543.5477
565.5296 601.5542
UltimateSPLASH™ ONE design strategy
C18:1 Ceramide-d7
50
569.5644 571.579
593.5609 629.5855
C20:1 Ceramide-d7
25
597.5957 599.6103
621.5922 657.6168
35:1
C22:1 Ceramide-d7
50
625.627 627.6416
649.6235 685.6481
17:0
PE 31:1 d5
C24:1 Ceramide-d7
75
653.6583 655.6729
677.6548 713.6794
O D DD D
O P
16:1 SM (d18:1/16:1)-d9 709.6084 C 39 H 68 D 9 N 2 O 6 P 75 18:1 SM (d18:1/18:1)-d9 737.6397 C 41 H 72 D 9 N 2 O 6 P 50 20:1 SM (d18:1/20:1)-d9 765.671 C 43 H 76 D 9 N 2 O 6 P 25 22:1 SM (d18:1/22:1)-d9 793.7023 C 45 H 80 D 9 N 2 O 6 P 50 24:1 SM (d18:1/24:1)-d9 821.7336 C 47 H 84 D 9 N 2 O 6 P 75
710.6157
768.6223
O D O O
O
33:1
37:3
HO
738.647
796.6536
NH 2
O
766.6783
824.6849
14:1
794.7096
852.7162
31:1
39.4
822.7409
880.7475
15:0 19:0 17:0
17:0-14:1 PC-d5
722.5622 C 39 H 71 D 5 NO 8 P 50 750.5935 C 41 H 75 D 5 NO 8 P 100 778.6248 C 43 H 79 D 5 NO 8 P 150 802.6248 C 45 H 79 D 5 NO 8 P 100 828.6405 C 47 H 81 D 5 NO 8 P 50 680.5153 C 36 H 65 D 5 NO 8 P 25 708.5466 C 38 H 69 D 5 NO 8 P 50 736.5779 C 40 H 73 D 5 NO 8 P 75 760.5779 C 42 H 73 D 5 NO 8 P 50 786.5935 C 44 H 75 D 5 NO 8 P 25 733.4918 C 37 H 65 D 5 NaO 10 P 25
723.5695
781.5761
17:0-16:1 PC-d5
751.6008
809.6074
14 16 18 20
30 32 34 36 38 40
17:0-18:1 PC-d5
779.6321
837.6387
17:0-20:3 PC-d5
803.6321
861.6387
Figure 1: Simulated RP separation of total acyl chain carbon for PE and LPE
17:0-22:4 PC-d5
829.6478
887.6543
Each unit of UltimateSPLASH™ ONE contains 69 high-purity, deuterium-labeled internal standards in a single vial of 1.2 mL of 1:1 DCM:MeOH. Each component has been meticulously characterized, quantitated, and blended for accurate quantitation using multiple lipidomics techniques.
17:0-14:1 PE-d5
679.508 681.5226
In addition to our easy to use mixture of all 69 internal standards in one vial, UltimateSPLASH™ internal standards are available in class specific mixtures, as well as individual internal standards. Details on ordering individual internal standards can be found on our website, along with the 15 separate class mixtures.
17:0-16:1 PE-d5
707.5393 709.5539
17:0-18:1 PE-d5
735.5706 737.5852
17:0-20:3 PE-d5
759.5706 761.5852
17:0-22:4 PE-d5
785.5863 787.6008
17:0-14:1 PG-d5
734.4991
751.5256 710.5026
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