SPLASH Brochure

MSI SPLASH TM Product number 330841

UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820

New product

As the role of lipids in neurodegenerative diseases such as Parkinson’s Disease is increasingly acknowledged, it has become essential to consider what lipids may have a hand in neuropathology. MSI SPLASH is made specifically for Mass Spec Imaging of neurological tissue. The mixture of thirteen internal standards is designed to be applied to brain sections using a sprayer, similarly to the application of matrix for MALDI. MSI SPLASH allows not only for quantitation of lipids within the brain, but also localization of those lipids to provide more detailed information for answering biological questions. This mixture was designed in collaboration with Michiel Vandenbosch, Shadrack Mutuku, Shane Ellis & Kim Ekroos. Toward Omics-Scale Quantitative Mass Spectrometry Imaging of Lipids in Brain Tissue Using a Multiclass Internal Standard Mixture. Michiel Vandenbosch, Shadrack M. Mutuku, Maria José Q. Mantas, Nathan H. Patterson, Tucker Hallmark, Marc Claesen, Ron M. A. Heeren, Nathan G. Hatcher, Nico Verbeeck, Kim Ekroos, and Shane R. Ellis. Analytical Chemistry 2023 95 (51), 18719-18730 DOI: 10.1021/acs.analchem.3c02724

Target conc (µg/mL)

Exact mass

Chemical formula

Compound name

(M-H) -

(M+H) +

(M-NH4) - (M+NH4) + (M-Na) -

(M+Na) + (M+AcO) -

17:0-16:1 PG-d5

761.5231 C 39 H 69 D 5 NaO 10 P 789.5544 C 41 H 73 D 5 NaO 10 P 813.5544 C 43 H 73 D 5 NaO 10 P 839.5701 C 45 H 75 D 5 NaO 10 P 746.4871 C 37 H 64 D 5 NaO 10 P 774.5184 C 39 H 68 D 5 NaO 10 P 802.5497 C 41 H 72 D 5 NaO 10 P 826.5497 C 43 H 72 D 5 NaO 10 P 852.5653 C 45 H 74 D 5 NaO 10 P 557.5068 C 34 H 59 D 5 O 5 585.5381 C 36 H 63 D 5 O 5 613.5694 C 38 H 67 D 5 O 5 637.5694 C 40 H 67 D 5 O 5 663.585 C 42 H 69 D 5 O 5 816.5525 C 40 H 73 D 5 NO 13 P 844.5838 C 42 H 77 D 5 NO 13 P 872.6151 C 44 H 81 D 5 NO 13 P 896.6151 C 46 H 81 D 5 NO 13 P 922.6307 C 48 H 83 D 5 NO 13 P 580.3384 C 24 H 45 D 5 NO 12 P 608.3697 C 26 H 49 D 5 NO 12 P 636.401 C 28 H 53 D 5 NO 12 P

50

762.5304

779.5569 738.5339

17:0-18:1 PG-d5

75

790.5617

807.5882 766.5652

17:0-20:3 PG-d5

50

814.5617

831.5882 790.5652

17:0-22:4 PG-d5

25

840.5773

857.6039 816.5808

17:0-14:1 PS-d5

25

745.4798 747.4943

723.4978

17:0-16:1 PS-d5

50

773.5111 775.5256

751.5291

17:0-18:1 PS-d5

75

801.5424 803.5569

779.5604

17:0-20:3 PS-d5

50

825.5424 827.5569

803.5604

Compound name

Exact mass Chemical formula Target conc (µM)

Target conc (µg/mL)

17:0-22:4 PS-d5

25

851.558 853.5726

829.5761

15:0-18:1 (d7) PA

689.499

C 39 H 69 D 5 NaO 10 P C 41 H 73 D 5 NaO 10 P C 43 H 73 DD 5 NaO 10 P C 45 H 75 DD 5 NaO 10 P C 37 H 64 D 5 NNaO 10 P C 39 H 68 D 5 NNaO 10 P C 41 H 72 D 5 NNaO 10 P C 43 H 72 D 5 NNaO 10 P C 45 H 74 D 5 NNaO 10 P

159.44

0.11

17:0-14:1 DG-d5

25

575.5406

580.496

17:0-16:1 DG-d5

50

603.5719

608.5273

15:0-18:1 (d7) PE

710.559

140.64

0.1

17:0-18:1 DG-d5

75

631.6032

636.5586

15:0-18:1 (d7) PG

763.536

64.14

0.049

17:0-20:3 DG-d5

50

655.6032

660.5586

15:0-18:1 (d7) PI

846.596

27.15

0.023

17:0-22:4 DG-d5

25

681.6188

686.5742

17:0-16:1 PS-d5

774.52

135.48

0.105

17:0-14:1 PI-d5

25

798.5186

17:0 Lyso PE-d5

472.33

6.35

0.003

17:0-16:1 PI-d5

50

826.5499

C12 Mono-sulfo galactosyl(beta) ceramide (d18:1/12:0)

740.486

25.64

0.019

17:0-18:1 PI-d5

75

854.5812

C15 Lactosyl(beta) ceramide-d7 (d18:1/15:0)

854.65

15.2

0.013

17:0-20:3 PI-d5

50

878.5812

C18 Ceramide-d7 (d18:1/18:0)

572.587

19.2

0.011

17:0-22:4 PI-d5

25

904.5969 940.6645

C17 Glucosyl(beta) Ceramide (d18:1/18:0)

713.581

C 34 H 59 D 5 O 5 C 36 H 63 D 5 O 5 C 38 H 67 D 5 O 5 C 40 H 67 D 5 O 5

186.26

0.133

15:0 Lyso PI-d5

25

562.3046 598.3723

SM d18:1/18:1(d9)

737.64

42

0.031

17:0 Lyso PI-d5

50

590.3359 626.4036

17:0 Lyso PC-d5

514.38

5.83

0.003

19:0 Lyso PI-d5

25

618.3672 654.4349

15:0-18:1 (d7) PC

752.606

213.79

0.161

15:0 Lyso PS-d5

510.273 C 21 H 36 D 5 NNaO 9 P 25 538.3043 C 23 H 40 D 5 NNaO 9 P 50 566.3356 C 25 H 44 D 5 NNaO 9 P 25 497.2778 C 21 H 37 D 5 NNaO 9 P 25 525.3091 C 23 H 41 D 5 NNaO 9 P 50 553.3404 C 25 H 45 D 5 NNaO 9 P 25

509.2658

487.2838

17:0 Lyso PS-d5

537.2971

515.3151

19:0 Lyso PS-d5

565.3284

543.3464

15:0 Lyso PG-d5

474.2886

17:0 Lyso PG-d5

502.3199

19:0 Lyso PG-d5

530.3512

15:0 Lyso PC-d5

486.3482 C 23 H 43 D 5 NO 7 P 514.3795 C 25 H 47 D 5 NO 7 P 542.4108 C 27 H 51 D 5 NO 7 P 444.3013 C 20 H 37 D 5 NO 7 P 472.3326 C 22 H 41 D 5 NO 7 P 500.3639 C 24 H 45 D 5 NO 7 P

25

485.341 487.3555

545.3621

17:0 Lyso PC-d5

50

513.3723 515.3868

573.3934

19:0 Lyso PC-d5

25

541.4036 543.4181

601.4247

15:0 Lyso PE-d5

25

443.294 445.3086

17:0 Lyso PE-d5

50

471.3253 473.3399

19:0 Lyso PE-d5

25

499.3566 501.3712

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