MSI SPLASH TM Product number 330841
UltimateSPLASH™ ONE ONE internal standard for lipidomic analysis Product number 330820
New product
As the role of lipids in neurodegenerative diseases such as Parkinson’s Disease is increasingly acknowledged, it has become essential to consider what lipids may have a hand in neuropathology. MSI SPLASH is made specifically for Mass Spec Imaging of neurological tissue. The mixture of thirteen internal standards is designed to be applied to brain sections using a sprayer, similarly to the application of matrix for MALDI. MSI SPLASH allows not only for quantitation of lipids within the brain, but also localization of those lipids to provide more detailed information for answering biological questions. This mixture was designed in collaboration with Michiel Vandenbosch, Shadrack Mutuku, Shane Ellis & Kim Ekroos. Toward Omics-Scale Quantitative Mass Spectrometry Imaging of Lipids in Brain Tissue Using a Multiclass Internal Standard Mixture. Michiel Vandenbosch, Shadrack M. Mutuku, Maria José Q. Mantas, Nathan H. Patterson, Tucker Hallmark, Marc Claesen, Ron M. A. Heeren, Nathan G. Hatcher, Nico Verbeeck, Kim Ekroos, and Shane R. Ellis. Analytical Chemistry 2023 95 (51), 18719-18730 DOI: 10.1021/acs.analchem.3c02724
Target conc (µg/mL)
Exact mass
Chemical formula
Compound name
(M-H) -
(M+H) +
(M-NH4) - (M+NH4) + (M-Na) -
(M+Na) + (M+AcO) -
17:0-16:1 PG-d5
761.5231 C 39 H 69 D 5 NaO 10 P 789.5544 C 41 H 73 D 5 NaO 10 P 813.5544 C 43 H 73 D 5 NaO 10 P 839.5701 C 45 H 75 D 5 NaO 10 P 746.4871 C 37 H 64 D 5 NaO 10 P 774.5184 C 39 H 68 D 5 NaO 10 P 802.5497 C 41 H 72 D 5 NaO 10 P 826.5497 C 43 H 72 D 5 NaO 10 P 852.5653 C 45 H 74 D 5 NaO 10 P 557.5068 C 34 H 59 D 5 O 5 585.5381 C 36 H 63 D 5 O 5 613.5694 C 38 H 67 D 5 O 5 637.5694 C 40 H 67 D 5 O 5 663.585 C 42 H 69 D 5 O 5 816.5525 C 40 H 73 D 5 NO 13 P 844.5838 C 42 H 77 D 5 NO 13 P 872.6151 C 44 H 81 D 5 NO 13 P 896.6151 C 46 H 81 D 5 NO 13 P 922.6307 C 48 H 83 D 5 NO 13 P 580.3384 C 24 H 45 D 5 NO 12 P 608.3697 C 26 H 49 D 5 NO 12 P 636.401 C 28 H 53 D 5 NO 12 P
50
762.5304
779.5569 738.5339
17:0-18:1 PG-d5
75
790.5617
807.5882 766.5652
17:0-20:3 PG-d5
50
814.5617
831.5882 790.5652
17:0-22:4 PG-d5
25
840.5773
857.6039 816.5808
17:0-14:1 PS-d5
25
745.4798 747.4943
723.4978
17:0-16:1 PS-d5
50
773.5111 775.5256
751.5291
17:0-18:1 PS-d5
75
801.5424 803.5569
779.5604
17:0-20:3 PS-d5
50
825.5424 827.5569
803.5604
Compound name
Exact mass Chemical formula Target conc (µM)
Target conc (µg/mL)
17:0-22:4 PS-d5
25
851.558 853.5726
829.5761
15:0-18:1 (d7) PA
689.499
C 39 H 69 D 5 NaO 10 P C 41 H 73 D 5 NaO 10 P C 43 H 73 DD 5 NaO 10 P C 45 H 75 DD 5 NaO 10 P C 37 H 64 D 5 NNaO 10 P C 39 H 68 D 5 NNaO 10 P C 41 H 72 D 5 NNaO 10 P C 43 H 72 D 5 NNaO 10 P C 45 H 74 D 5 NNaO 10 P
159.44
0.11
17:0-14:1 DG-d5
25
575.5406
580.496
17:0-16:1 DG-d5
50
603.5719
608.5273
15:0-18:1 (d7) PE
710.559
140.64
0.1
17:0-18:1 DG-d5
75
631.6032
636.5586
15:0-18:1 (d7) PG
763.536
64.14
0.049
17:0-20:3 DG-d5
50
655.6032
660.5586
15:0-18:1 (d7) PI
846.596
27.15
0.023
17:0-22:4 DG-d5
25
681.6188
686.5742
17:0-16:1 PS-d5
774.52
135.48
0.105
17:0-14:1 PI-d5
25
798.5186
17:0 Lyso PE-d5
472.33
6.35
0.003
17:0-16:1 PI-d5
50
826.5499
C12 Mono-sulfo galactosyl(beta) ceramide (d18:1/12:0)
740.486
25.64
0.019
17:0-18:1 PI-d5
75
854.5812
C15 Lactosyl(beta) ceramide-d7 (d18:1/15:0)
854.65
15.2
0.013
17:0-20:3 PI-d5
50
878.5812
C18 Ceramide-d7 (d18:1/18:0)
572.587
19.2
0.011
17:0-22:4 PI-d5
25
904.5969 940.6645
C17 Glucosyl(beta) Ceramide (d18:1/18:0)
713.581
C 34 H 59 D 5 O 5 C 36 H 63 D 5 O 5 C 38 H 67 D 5 O 5 C 40 H 67 D 5 O 5
186.26
0.133
15:0 Lyso PI-d5
25
562.3046 598.3723
SM d18:1/18:1(d9)
737.64
42
0.031
17:0 Lyso PI-d5
50
590.3359 626.4036
17:0 Lyso PC-d5
514.38
5.83
0.003
19:0 Lyso PI-d5
25
618.3672 654.4349
15:0-18:1 (d7) PC
752.606
213.79
0.161
15:0 Lyso PS-d5
510.273 C 21 H 36 D 5 NNaO 9 P 25 538.3043 C 23 H 40 D 5 NNaO 9 P 50 566.3356 C 25 H 44 D 5 NNaO 9 P 25 497.2778 C 21 H 37 D 5 NNaO 9 P 25 525.3091 C 23 H 41 D 5 NNaO 9 P 50 553.3404 C 25 H 45 D 5 NNaO 9 P 25
509.2658
487.2838
17:0 Lyso PS-d5
537.2971
515.3151
19:0 Lyso PS-d5
565.3284
543.3464
15:0 Lyso PG-d5
474.2886
17:0 Lyso PG-d5
502.3199
19:0 Lyso PG-d5
530.3512
15:0 Lyso PC-d5
486.3482 C 23 H 43 D 5 NO 7 P 514.3795 C 25 H 47 D 5 NO 7 P 542.4108 C 27 H 51 D 5 NO 7 P 444.3013 C 20 H 37 D 5 NO 7 P 472.3326 C 22 H 41 D 5 NO 7 P 500.3639 C 24 H 45 D 5 NO 7 P
25
485.341 487.3555
545.3621
17:0 Lyso PC-d5
50
513.3723 515.3868
573.3934
19:0 Lyso PC-d5
25
541.4036 543.4181
601.4247
15:0 Lyso PE-d5
25
443.294 445.3086
17:0 Lyso PE-d5
50
471.3253 473.3399
19:0 Lyso PE-d5
25
499.3566 501.3712
www.avantiresearch.com
4
5
Made with FlippingBook - professional solution for displaying marketing and sales documents online