SPLASH Brochure

Oxysterol SPLASH TM Product number 330700

SPLAS H TM LIPIDOMIX TM Mass Spec Standard Product number 330707

Best seller

Oxysterols are intermediates in the biosynthesis of both bile acids and steroids, but some may also have their own biological activities in vivo. Conditions such as Alzheimer’s Disease, Multiple Sclerosis, and various cancers may be connected to altered oxysterol levels as well. OxysterolSPLASH TM contains 13 deuterated oxysterols at equal concentrations, making the mixture a useful tool for lipidomic analysis of these compounds.

The original SPLASH TM internal standard mixture has been a best-seller since it was first developed in 2016. It contains one deuterium labeled internal standard for each of 14 lipid classes at ratios relative to human plasma lipids. Each vial contains 1mL of internal standard mixture in methanol solution with a suggested sample spiking ratio of 10μL SPLASH TM to 10μL human plasma, allowing for up to 100 samples to be analyzed with one vial. Concentrations are verified for use as a quantitative standard.

Compound name

Exact mass

Chemical formula Target conc (µM)

Target conc (µg/mL)

Compound name

Exact mass

Chemical formula Target conc (µg/mL)

15:0-18:1(d7) PC

752.61

C 41 H 73 D 7 NO 8 P C 38 H 67 D 7 NO 8 P

160

213

24(R/S)-hydroxycholesterol-d7

409.394

C 27 H 39 D 7 O 2 C 27 H 40 D 6 O 2 C 27 H 40 D 6 O 2 C 27 H 39 O 2 D 7 C 27 H 39 O 2 D 7 C 27 H 37 O 2 D 7 C 27 H 37 D 7 O 2 C 27 H 40 D 6 O 3 C 27 H 40 D 6 O 3 C 27 H 39 D 3 O 4 C 27 H 39 O 2 D 7 C 27 H 39 O 2 D 7 C 27 H 41 D 7 O 3

150

5

8

15:0-18:1(d7) PE

710.56

25-hydroxycholesterol-d6

408.387

150

C 39 H 66 D 7 NNaO 10 P C 39 H 67 D 7 NaO 10 P

5

5

15:0-18:1(d7) PS (Na Salt)

776.53

27-hydroxycholesterol-d6

408.387

150

30

38

15:0-18:1(d7) PG (Na Salt)

763.54

7α-hydroxycholesterol-d7

409.394

150

C 42 H 75 D 7 NO 13 P C 36 H 61 D 7 NaO 8 P C 26 H 45 D 7 NO 7 P C 23 H 39 D 7 NO 7 P

10

11

15:0-18:1(d7) PI (NH4 Salt)

846.6

7ß-hydroxycholesterol-d7

409.394

150

7

11

15:0-18:1(d7) PA (Na Salt)

689.5

7-ketocholesterol-d7

407.378

150

25

48

18:1(d7) Lyso PC

528.39

7α-hydroxycholestenone-d7

407.378

150

5

11

18:1(d7) Lyso PE

486.35

7α,25-dihydroxycholesterol-d6

424.382

150

C 45 H 71 D 7 O 2 C 21 H 33 D 7 O 4 C 36 H 61 D 7 O 5 C 51 H 89 D 7 O 6

350

541

18:1(d7) Chol Ester

657.64

7α,27-dihydroxycholesterol-d6

424.382

150

2

6

18:1(d7) MAG

363.34

7α-Hydroxy-3-oxocholest-4-enoic acid-d3

433.33

150

10

16

15:0-18:1(d7) DAG

587.55

55

71

15:0-18:1(d7)-15:0 TAG

811.77

4ß-hydroxycholesterol-d7

409.394

150

C 41 H 72 D 9 N 2 O 6 P

30

42

d18:1-18:1(d9) SM

737.64

22(R)-hydroxycholesterol-d7

409.394

150

C 27 H 39 OD 7

100

248

Cholesterol (d7)

393.4

5α,6ß-dihydroxycholestanol-d7

427.404

150

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