Chen 2009 | Tissue repair
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Chen CH, Tsai JL, Wang YH, Lee CL, Chen JK, Huang MH. Low-level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine Achilles tendon fibroblasts in vitro. J Orthop Res. 2009 May;27(5):646-50.
Laboratory study Methods : 4 groups of identically cultured fibroblasts were exposed to LLLI and harvested after 24 h. • Group 1 (control group) received no LLLI.
• Group 2 received 1 J/cm2 LLLI • Group 3 received 2 J/cm2 LLLI • Group 4 received 3 J/cm2 LLLI Results :
• When compared to the control group, the cell proliferation of irradiated AT fibroblasts in the other 3 groups increased significantly by 13% (Group 2), 30% (Group 3), and 12% (Group 4) respectively. • The mRNA expressions of decorin and type I collagen in fibroblasts with LLLI were significantly higher than in the control condition (p < 0.05).
Low level laser irradiation promotes cell proliferation and production of mRNA for type I collagen and decorin synthesis.
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