Torricelli et al. 2001 | Cartilage repair
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Torricelli P, Giavaresi G, Fini M, Guzzardella GA, Morrone G, Carpi A, Giardino R. Laser biostimulation of cartilage: in vitro evaluation. Biomed Pharmacother. 2001 Mar;55(2):117-20.
In vitro study . Methods : Chondrocyte cultures were derived from rabbit and human cartilage. • These cells were exposed to laser treatment for 5 days, using the following parameters: 300 joules, 1 watt, 100 (treatment A) or 300 (treatment B) hertz, pulsating emission for 10 minutes, under a sterile laminar flow. • Control cultures (no treatment) received the same treatment with the laser device off. Outcome : Cell viability was measured by MTT assay at the end of the laser treatment and then after 5 days. Results: • Neither rabbit nor human cultured chondrocytes showed any damage under a light microscope and immunostaining control following laser treatment. • The MTT test results indicated a positive biostimulation effect on cell proliferation with respect to the control group. • The increase in viability of irradiated chondrocytes was maintained for five days following the end of the laser treatment.
Laser irradiation had a positive biostimulation effect on chondrocyte cell proliferation which was maintained for five days following the laser treatment.
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