The Avanti Research Mini-Extruder brochure presents a compact and cost-effective tool for preparing unilamellar vesicles by extrusion. The Mini-Extruder is designed for ease of use, allowing rapid production of small or large unilamellar vesicles without solvents. The system includes features such as a heating block for temperature control, compatibility with various lipid compositions, and interchangeable syringe options. The brochure highlights the device’s reliability, flexibility, and affordability for researchers needing precise vesicle production. For more details, you can view the full brochure here.
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The Avanti Research Mini-Extruder
Convenient
Flexible
Reliable
The particle size distribution of unilamellar vesicles prepared by extrusion is a function of the number of passes through the extruder membrane. Hydrated lipid films will initially form large, multi-lamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids.
• Prepare small or large, unilamellar vesicles (SUVs or GUVs) by extrusion in an easy, rapid, & efficient manner • Design allows for the rapid cleaning of all wetted parts, reducing down-time between production of vesicles from batch to batch. • Generate uniform populations of unilamellar vesicles without the use of solvents or detergents • Vesicle size readily controlled by polycarbonate membrane selection
• Optional heating block allows the extrusion of vesicles at elevated temperatures, which is critical for the successful production of vesicles from phospholipids with a phase transition temperature above room temperature • Generate unilamellar vesicles ranging from 30nm – 1µm when assembled with appropriate polycarbonate membrane • Useful for a wide variety of lipid compositions • Interchangeable with 250µL & 1000µL syringes
Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.
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Initial particle size distribution
Affordable
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O-ring channel
Filter supports
Extruder washer - PTFE
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O-ring
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• Available for a fraction of the cost of a larger extruder • All replacement parts and accessories are available from Avanti • Complete set includes: • Mini-extruder • Heating block/holder • 2-Gas Tight Syringes (1000uL) • 2 O-rings • 100 polycarbonate membranes (0.1µm) • 100 Polyester filter supports
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3 passes through 100nm membrane
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Retainer nut
11 passes through 100nm membrane
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Non-warranty The information in this publication is believed to be accurate and is given in good faith, but no representation or warranty as to its completeness or accuracy is made. Suggestions for uses or applications are only opinions. Users are responsible for determining the suitability of these products for their own particular purpose. No representation or warranty, expressed or implied, is made with respect to information or products including, without limitation, warranties of merchantability, fitness for a particular purpose, non-infringement of any third-party patent or other intellectual property rights including, without limit, copyright, trademark and designs. Unless otherwise stated, any trademarks identified herein are trademarks of the Croda group of companies. Avanti Research is a trademark of Croda international Plc. Avanti Research is a Croda brand associated with Avanti Polar Lipids, LLC.
©2024 Croda International.
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Pharma
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