The Avanti Research Mini-Extruder
Convenient
Flexible
Reliable
The particle size distribution of unilamellar vesicles prepared by extrusion is a function of the number of passes through the extruder membrane. Hydrated lipid films will initially form large, multi-lamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids.
• Prepare small or large, unilamellar vesicles (SUVs or GUVs) by extrusion in an easy, rapid, & efficient manner • Design allows for the rapid cleaning of all wetted parts, reducing down-time between production of vesicles from batch to batch. • Generate uniform populations of unilamellar vesicles without the use of solvents or detergents • Vesicle size readily controlled by polycarbonate membrane selection
• Optional heating block allows the extrusion of vesicles at elevated temperatures, which is critical for the successful production of vesicles from phospholipids with a phase transition temperature above room temperature • Generate unilamellar vesicles ranging from 30nm – 1µm when assembled with appropriate polycarbonate membrane • Useful for a wide variety of lipid compositions • Interchangeable with 250µL & 1000µL syringes
Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.
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90
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Initial particle size distribution
Affordable
30
O-ring channel
Filter supports
Extruder washer - PTFE
20
O-ring
O-ring
• Available for a fraction of the cost of a larger extruder • All replacement parts and accessories are available from Avanti • Complete set includes: • Mini-extruder • Heating block/holder • 2-Gas Tight Syringes (1000uL) • 2 O-rings • 100 polycarbonate membranes (0.1µm) • 100 Polyester filter supports
10
3 passes through 100nm membrane
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Time (minutes
Heating block temperature
Syringe temperature
Extruder outer casing
Internal membrane support
Polycarbonate membrane
Internal membrane support
Retainer nut
11 passes through 100nm membrane
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10
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1000 7000
Size (nm)
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