QUARTERLY BEAT / JULY 2024
TABLE 1. DONOR REQUIREMENTS/SCREENING
DONORS SHOULD FULFILL THESE CRITERIA
FECAL SCREENING
EXCLUDE
· Normal dysbiosis index · Salmonella spp
· 12 months to middle-age · Body condition score of 4-6/9 · Healthy, no medication · CIBDAI ≤ 3 (dogs) · No antibiotics for 6 months (minimum) · Not being fed a raw food diet · Normal haematology and serum biochemistry · Cats: indoor, preferably single-cat household · Negative for enteric coronavirus (PCR) · FIV and FeLV negative
· Campylobacter jejuni · Giardia and other intestinal parasites · In cats also Tritrichomonas foetus
Consensus is lacking on the FMT dose in veterinary medicine. The amount of donor stool per treatment has varied in different studies. The author uses 5 grams of donor faeces per kg body weight (BW) of the recipient for dogs up to 25 kg BW and 3 grams of faeces per kg BW for dogs > 25 kg. In dogs with CE, 3 FMTs with 10-20 days intervals are given as one set of treatments. If clinical improvement is lacking after FMT 1 or 2, we do not give a 3rd treatment, based on our previous results. 8
We use fresh frozen faeces, stored at -20 °C for a maximum of 3 months, without cryopreservatives. Cryopreservatives increase the number of viable bacteria in frozen faeces, but whether or not adding cryopreservatives, such as 10 % glycerol, actually makes a clinical difference has not been studied. Fresh frozen faeces without cryopreservatives has been used with a good outcome in several of the published studies on FMT in dogs to date. 5,6,8
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