ICCFGG 2022
#44 Heritability and genome-wide association study of dog behavioral phenotypes in a commercial breeding cohort
N. Bhowmik1 , S. Cook1, C. Croney, S. Barnard2, A. Romaniuk2, K.J. Ekenstedt1 nbhowmik@purdue.edu
1Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA, 2Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA Canine behavior is an important component in the success of the human-dog relationship, and in the dog’s overall welfare, making selection for behavior an important part of any breeding program. While behaviors are complex traits controlled by many genes of small effects, the environment, and gene×environment interactions, genetic selection for desirable behavioral phenotypes remains possible. No genomic association studies of dog behavior to date have reported on a commer- cial breeding (CB) cohort, therefore, dogs from these facilities were utilized. The validated Field Instantaneous Dog Observation tool was used to assign seven behavioral test score phenotypes, and one comprehensive score, to 615 dogs representing 52 breeds and four breed crosses, which were genotyped on the 710K Affymetrix SNP array. Once corrected for facility, breed, sex, and age, heritability estimates for individual phenotypes ranged from 0.094±0.077 to 0.340±0.164, and 0.338±0.114 for the comprehensive score. Genome-wide association analysis, adjusted for facility, principal component 1, body size, and a kinship matrix, identified seven markers associated with the comprehensive behavioral phenotype (p < 1 × 10-5). Thirty-eight genes were identified within a 1Mb flanking region of each marker. Six genes (PRNP, DEPDC7, ADRA1D, ALDH1A2, CSTF3, and TCP11L1) were associated with anxiety, depression, aggression, fearfulness, and angry temper- ament in humans and rat. Three genes (HIPK3, SLC23A2, and TCF12) were previously associated with canine phenotypes: dog and stranger aggression, dog fear, rivalry, and separation problems. This study confirms the high genetic similarities between genetic behavior phenotypes in this CB cohort and populations used in previous studies.
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