POSTER ABSTRACTS
#45 Clustering analysis of lipoprotein profiles to identify subtypes of primary hyperlipidemia in Miniature Schnauzers Nicole M Tate1 , Panagiotis G Xenoulis2,3, Joerg M Steiner3, Punyamanee Yamkate3, Erica L Behling-Kelly4, Aaron K Rendahl5, and Eva Furrow1 nmtate@umn.edu 1Department of Veterinary Clinical Sciences, University of Minnesota, College of Veterinary Medicine, St. Paul, MN, USA, 2Clinic of Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece, 3Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A & M University, College Station, TX, USA, 4Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 5Department of Veterinary and Biomedical Sciences, University of Minnesota, College of Veterinary Medicine, St. Paul, MN, USA Primary hyperlipidemia is prevalent in Miniature Schnauzers, predisposing them to life-threatening diseases. Varied responses to management strategies suggest the possibility of multiple subtypes. The identification of genetic risk factors relies on accurate phenotyping. The aim of this study is to identify potential subtypes of hyperlipidemia in Miniature Schnauzers using continuous lipoprotein density profiling and cluster analysis. We hypothesize that multiple hyperlipidemia phenotypes exist. Lipoprotein profiles were generated for 20 Miniature Schnauzers with normal serum tri- glyceride concentrations (NTG), 25 with primary hypertriglyceridemia (HTG), and 5 with secondary HTG. Clinical data (age, sex, body condition score, and dietary fat) was compared between clusters. Six clusters were identified. Three clusters comprised primarily HTG dogs. One showed the highest intensities for triglyceride-rich lipoprotein (TRL) and low-density lipoprotein (LDL) fractions. The second showed moderately increased TRL fraction intensities with intermediate intensities across other fractions. The third showed the lowest LDL fraction intensities and intermediate TRL fraction intensities; HTG cases in this cluster were mild. Two clusters comprised only NTG dogs with lower TRL intensities and low-to-intermediate LDL intensities. The remaining cluster included a mix of NTG and mild HTG dogs with increased LDL but variable TRL fraction intensities. The clinical data was not a significant source of differences between clusters. The results support a spectrum of lipoprotein phenotypes that cannot be predicted by triglyceride concentration alone. This study highlights the importance of distinguishing subtypes of a disease, which could have major implica- tions in genetic investigations.
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